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This outstanding program provides a solid foundation in molecular genetics and will prepare your students for advanced college-level courses. Your students will investigate such contemporary topics as DNA electrophoresis, restriction nuclease mapping, and molecular cloning procedures.
Experiments in this program:
EXP-301 Length of the DNA Molecule:
Electrophoresis in agarose gels is the most common method used for determining the size of DNA molecules. In this introductory exercise, students determine the length of an unknown DNA molecule by comparing its electrophoretic mobility with 11 DNA molecules of known size (a standardized ladder).
EXP-302 Restriction Nuclease Mapping of DNA:
Bacteriophage lambda is a DNA virus that attacks E. coli. Here, students dissect lambda DNA using the restriction endonucleases EcoR1 and BamH1 in order to identify specific sites, sequences, and structures along the phage genome. This single exercise enables students to explore a number of exciting topics in molecular biology, including the specificity of restriction endonucleases, DNA mapping strategies, complementary base-pairing of DNA, and the structure of a viral genome.
EXP-304A Molecular Cloning:
In part A of Molecular Cloning, students create a strain of E. coli that is resistant to the antibiotic ampicillin by introducing a plasmid that contains an ampicillin-resistance gene. The success of the transformation is monitored by growing the bacteria on an ampicillin-containing media. This experiment provides sufficient sterile materials for sixteen platings.
This program also includes:
- 5g DNA-Electrophoresis-Grade Agarose
- 50ml of 100x Electrophoresis Buffer, enough to make 5L
- 5ml of 1000x Methylene Blue Gel Stain for viewing DNA after electrophoresis
- 3ml of 700x Toluidine Blue Pre-Stain for viewing DNA in-gel as electrophoresis proceeds
- USB Flash Drive containing student and instructor protocols for all included experiments