IND-14: Enzyme Kinetics

Includes instructions, wheat germ, acid phosphatase, extraction buffer, acid phosphatase substrate, substrate dilution buffer, nitrophenol standards, small transfer pipets and large transfer pipets.

IND-16: Identifying Viral DNA by Rapid Southern Blotting

Includes instructions, DNA samples 1-3, (instructor keyed),dye mixture, blotting paper, nylon membranes, petri dishes, methylene blue, Tris-buffer saline (TBS), TBS+NP-40, streptavidin peroxidase, Hydrogen peroxide, chloronapthol, gelatin and Tris buffer.

IND-21: Identifying Genomic and Plasmid DNA Sequences in E.coli by Colony PCR

Includes instructions, E. coli-pUC 18, nutrient agar-ampicillin, ampicillin, petri dishes, inoculating loops, ampicillin-resistance gene primers, rDNA primers, sterile pipet tips, PCR buffer,Taq polymerase, Taq dilution buffer, nucleotides, DNA size markers, electrophoresis sample buffer, mineral oil, and PCR tubes.

IND-25P: Isolation of Chromosomal Proteins

Includes chicken erythrocytes, standard proteins, sample buffer, nuclear isolation buffer, micrococcal nuclease and instructions. Requires two 12 well gels for 8 groups of students.

IND-26: Localizing Tublin by Immunohistochemistry

Microtubules are hollow cylinders made up of polymers of the protein tubulin. Microtubules are major components of cilia and flagella, which are tail like projections that are covered by a plasma membrane and extend outwards from the cell. Motile cilia are used for locomotion and food gathering by some protozoa and are found in the […]

IND-27: Composition of the Nucleosome

Includes detailed instructions, chicken erythrocytes, prestained standard proteins, protein sample buffer, DNA sample buffer, calf thymus nucleosome standard DNA, nuclear isolation buffer, micrococcal nuclease, nuclear stain, EDTA, small transfer pipets and large transfer pipets.

S1: Introduction to Molecular Biology

The complete Package for 16 students working in pairs plus one student manual and one instructor manual (The student manual may be reproduced for educational purposes.)