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Description

This Program is a complete laboratory course for teaching cell biology or introductory molecular biology at the college level. The program is designed for 16 students working in pairs and provides essentially all of the chemicals and instructions that are needed to teach sixteen 2-3 hour laboratory sessions. The course consists of 14 experiments carefully selected from Standard Programs 1, 2, 3, and 7 that are presented in a comprehensive integrated laboratory manual.

The Table of Contents of the manual and the catalog numbers of the experiments provided with the program are shown below. To use this program, you will need an Accessory Kit, the appropriate electrophoresis equipment, microscopes, and a water bath. A small centrifuge is required for 2 experiments. The price of the course is about $51 per student per semester if these items are available in your teaching laboratory.

In this laboratory course, your students will be introduced to the molecular biology of the eukaryotic cell. In the first section of the course, students study topics in protein biology and biochemistry such as protein structure, function, isolation, molecular evolution, and the detection and molecular basis of human disease. Techniques used for these experiments include electrophoresis (both native and denaturing), affinity chromatography, peptide mapping, and the Western blot procedure. In the second section of the course, students localize enzymes in plant and animal cells, perform cell fractionation procedures, and study the properties of a specific cell-surface receptor. Experiments on the properties and structure of DNA are presented in the final section of the course. Techniques include DNA electrophoresis and restriction nuclease mapping.

Features

Features:

  • Suitability – Eukaryotic Cell Biology or Introductory Molecular Biology for sophomores and juniors.
  • Lab Schedule – One 2-3 hour lab session per week for a full semester.
  • Cost – $51 per student per semester excluding costs of manuals and equipment.

Experiments

The experiments that comprise this course were selected from four Programs: