A first step in characterizing a protein often involves determining its molecular weight. From this information, different proteins may be compared and the number of amino acid residues in a protein can be determined. Here, students determine the molecular weight of two unknown proteins by comparing their electrophoretic migration with the migration of standard proteins. The protein standards and unknowns have been pre-stained so that your students can follow their progress during the separation as shown.
Each of the individual experiments is supplied with the chemicals and laboratory guides needed for 16 students working in pairs.
If ordering the EXP-201P variant, precast polyacrylamide gels are not included. Precast polyacrylamide gels can be ordered from Bio Rad, (800-424-6723) Their catalog number is 4561093EDU.