Teh reagents in this kit are used to isolate DNA from bacteria or eukaryotic tissues. The unique procedure yields high quality genomic DNA with the use of toxic organic solvents. The procedure has been successfully used to isolate DNAs from E.coli, corn seedlings, wheat germ, chicken erythrocytes, and calf thymus. In each case, the DNAs were undergraded and were excellent substrates for a variety of restriction endonucleases. Kit includes NB-buffer, SDS, ammonium acetate, Tris-EDTA buffer, an electrophoresis buffer containingribonuclease and wheat germ so that you can test the procedure. Ethyl alcohol and a centrifuge that can be operated at a force of at least 5000 x g are needed but are not provided.