This Program is a complete laboratory course for teaching molecular biology or biochemistry at the advanced college level. The program is designed for up to 16 students working in pairs and provides essentially all of the chemicals and instructions that are needed to teach twenty eight 2-3 hour laboratory sessions. The course consists of experiments carefully selected from the Standard Programs that are presented in a comprehensive integrated laboratory manual. The Table of Contents of the manual and the catalog numbers of the experiments provided with the program are shown below. To use this program, you will need an Accessory Kit, the appropriate electrophoresis equipment, microscopes, a water bath for DNA hybridization, and a centrifuge that can be operated at a force of at least 3000 x g. A bacterial shaker is also strongly recommended. The price of the course is about $77 per student per semester if these items are available in your teaching laboratory.
In this laboratory course, your students will be introduced to many of the concepts and techniques that have revolutionized the biological sciences during the past two decades. In the first section of the course, students study topics in protein biology and biochemistry such as protein structure, function, isolation, location in cells, molecular evolution, and the detection and molecular basis of human disease. Techniques used for these experiments include electrophoresis (both native and denaturing), affinity chromatography, peptide mapping, enzyme cytochemistry and the Western blot procedure. In the second section of the course, students perform experiments with DNA structure and function. These exercises stress the organization and complexity of the prokaryotic and eukaryotic genomes, gene function and regulation, and the structure of the eukaryotic chromosome. Techniques include restriction nuclease mapping, Southern blot hybridization procedures, and an array of state-of-the-art DNA cloning techniques.