Description
In this experiment, students prepare a protein extract from cow heart. They then determine the molecular weights of major contractile proteins by comparing their migration on SDS-polyacrylamide gels to the migration of standard proteins of known size. Students also identify and determine the molecular weight of the major proteins found in milk. This exercise requires 1 three-hour or 2 two-hour lab sessions and a table top centrifuge or microcentrifuge is required. Typical results of the exercise are shown on the gel.
Precast polyacrylamide gels are not included. Precast polyacrylamide gels can be ordered from Bio Rad, (800-424-6723) Their catalog number is 4561093EDU.