Vitellogenin is a protein produced in hen liver under the influence of estrogen. This sex-specific protein enters the circulatory system and is transported to the ovaries where it is broken down into the egg yolk proteins lipovitellin and phosvitin. In this exercise, students compare the proteins in egg yolk and in hen and rooster sera by electrophoresis and identify vitellogenin, lipovitellin and phosvitin. This exercise illustrates the use of electrophoresis for protein identification and introduces concepts in molecular endocrinology.
Each of the individual experiments are supplied with the chemicals and laboratory guides needed for 16 students working in pairs. If you chose one or more of the experiments from this series – you should also orderElectrophoresis Package 2M or Electrophoresis Package 2. Electrophoresis Package 2M provides sufficient agarose, gel stain, and electrophoresis buffers for 1 of the individual experiments in this series (four gels with 15ml of agarose per gel). Electrophoresis Package 2 provides sufficient agarose, gel stain, and electrophoresis buffers for up to 6 of the individual experiments in this series.