B1-3: Gene Function and Cloning in Bacteria

Occasionally, E. coli cells are found in nature that are resistant to the toxic effects of the antibiotic ampicillin. In this exercise, students create such an ampicillin-resistant population of E. coli by introducing into bacterial cells a plasmid that contains an ampicillin-resistance gene. Sufficient sterile materials are supplied for sixteen platings. The introduction of plasmid DNA into bacterial cells is called transformation and has enabled scientists to obtain large quantities of more than 1000 genes including those for human interferon, insulin, and growth hormone.

IND-09: Producing a Strain of E. coli that Glows in the Dark

Includes instructions, E.coli, plasmid lux, control plasmid, calcium chloride, inoculating loops, transfer pipets, tubes, petri dishes, ampicillin,nutrient agar-ampicillin, and nutrient broth. All materials are provided sterile and ready for use.

S10: The New Genetics

The Complete Package for 16 students working in pairs plus one student manual and one instructor manual (The student manual may be reproduced for educational purposes).

S11: Cloning a DNA Segment

The Complete Package for 16 students working in pairs plus one student manual and one instructor manual (The student manual may be reproduced for educational purposes.)