B3: Chromatographic Separation of Proteins

Gel filtration is a chromatographic method used to separate molecules of different sizes. This technique is an important tool for isolating and characterizing proteins. In this series of experiments, students use this method along with basic enzyme and antibody assays to study the structure and function of proteins.

B4-2: Extraction and Analysis of an Enzyme from Wheat

Acid phosphatase is present in many plant tissues where it catalyzes the removal of phosphate groups from macromolecules at low pH. In this exercise, students prepare a cell-free extract from wheat germ and determine the amount of the enzyme present in the extract. The experiment offers practical experience with enzyme extraction procedures and is an […]

EXP-401: Evolution of the Vertebrate Genome

The history of evolution is recorded in the genomes of present-day organisms and enlightened guesses of evolutionary events can be made by comparing DNA sequences in different species. Evidence drawn from this approach has led to the construction of family trees of organisms that agree remarkably well with those obtained from more traditional procedures. In […]