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An Introduction to Molecular Genetics (O2)

This outstanding program provides a solid foundation in molecular genetics and will prepare your students for advanced college-level courses. Your students will investigate such contemporary topics as DNA electrophoresis, restriction nuclease mapping, and molecular cloning procedures. We now offer a video which was designed to be used with this program. Overview 2 contains the following experiments selected from Standard Program 3:

EXP-301 301. The Length of DNA Molecules (View Individual Experiment)

Electrophoresis in agarose gels is the most common method used for determining the size of DNA molecules. In this introductory exercise, students determine the length of an unknown DNA molecule by comparing its electrophoretic mobility with six DNA molecules of known size as shown.

EXP-302 302. Restriction Nuclease Mapping of DNA (View Individual Experiment)

Bacteriophage lambda is a DNA virus that attacks E. coli. Here, students dissect lambda DNA using the restriction endonucleases EcoR1 and BamH1 in order to identify specific sites, sequences, and structures along the phage genome. This single exercise enables students to explore a number of exciting topics in molecular biology, including the specificity of restriction endonucleases, DNA mapping strategies, complementary base-pairing of DNA, and the structure of a viral genome.

DNA STAINING PROCEDURE

EXP-304 304. Molecular Cloning (View Individual Experiment)

This two part exercise provides state-of-the-art information and practical experience with a variety of techniques that form the foundation of the biotechnology industry. In part A, students create a strain of E. coli that is resistant to the antibiotic ampicillin by introducing a plasmid that contains an ampicillin-resistance gene. The success of the transformation is monitored by growing the bacteria on an ampicillin-containing media. This experiment provides sufficient sterile materials for sixteen platings.

Prices

Catalog # Price Description
O2 188.00

The Chemical Package for 16 students working in pairs plus 17 student manuals and one instructor manual.
O2-C 137.06

The Chemical Package for 16 students working in pairs plus one student manual and one instructor manual (The student manual may be reproduced for educational purposes.)
O2-SM 6.66

Sample Student Manual (36 pages) plus one instructor manual.