An Introduction to Electrophoresis (O3)

This series of three experiments provides an introduction to agarose gel electrophoresis, In the first experiment, students identify unknown dye molecules by comparing the electrophoresis migration with the migration of known dyes. In the second, they identify dye molecules that bind to DNA and determine the mechanism. They then study four proteins in experiment three and relate differences in they charges to their migration rates in an electric filed. Each protein is a different color so that it's progress during the separation can easily be followed, The experiments were designed for 8 groups of students and the package includes four colorful dye mixtures, four colored proteins, DNA, agarose, electrophoresis buffer, gel loading transfer pipets and complete instructions. Each experiment can be completed within a 2-hour laboratory period, The exercises in the program are: