This collection of experiments was prepared for the novice to help bridge the gap between traditional animal physiology and the biochemistry of cell processes in animals. Following the step-by-step procedures in their laboratory manuals, students will study a variety of topics including the action, properties, and cell location of enzymes, and the evolution of mammalian proteins.
This mini program is composed of Experiments 1-3 from Modern Biology's Basic Program 3.
1. Separating Molecules by Gel Filtration
Students fill their chromatographic columns with packing material and separate three colored molecules of known molecular weights as shown in the photograph below. The exercise illustrates how components of a complex mixture can be separated by chromatography and introduces the concept of the standard curve for determining the size of an unknown protein.
The program contains selected experiments from Standard Program 1 and stresses the relevance of molecular biology to the study of human genetics and disease. The experiments provide an introduction to electrophoresis and to protein composition, structure, and function in health and disease. The exercises in the program are:
This program utilizes 6 experiments to stress the relevance of molecular biology and to the study of physiology and human disease. Students first learn how proteins are separated on the basis of charge by electrophoresis. With this information, they perform a series of experiments aimed at illustrating the importance of the technique in biology and medicine. Students examine proteins in biological fluids and relate their findings to physiological and pathological states.
The sheep genome contains a highly repeated satellite sequence. In this program, students use recombinant DNA techniques to clone this satellite DNA and then characterize the sequence by restriction enzyme mapping anaylsis. The program provides essentially all of the instructions, chemicals, sterile media, and expendable accessories that are needed to carry out DNA electrophoresis, restriction nuclease digestion, DNA ligations, bacterial transformations, bacterial selection for ampicillin resistance and ß - glactosidase production and plasmid isolation.