This exercise was designed to provide an exciting introduction to specific gene structure and function. In the experiment, students are given two plasmids (A and B) which are identified in the instructors guide. One plasmid (A) has a functional gene for the enzyme ß-galactosidase. The ß-galactosidase gene in the other plasmid (B) is inactive because it contains a segment of foreign DNA. In the first part of the exercise, students analyze restriction digests of both plasmids in order to determine which plasmid should have a functional ß-galactosidase gene.
Microtubules are hollow cylinders made up of polymers of the protein tubulin. Microtubules are major components of cilia and flagella, which are tail like projections that are covered by a plasma membrane and extend outwards from the cell. Motile cilia are used for locomotion and food gathering by some protozoa and are found in the lining of the trachea, where their wave like motion propels mucus, dust and other foreign matter out of the lungs.
Synthetic biology is an exciting new field that uses engineering principles and mathematical modeling to design and construct biological devices. Synthetic biology projects include the construction of bacterial computers that can solve mathematical problems. Microbial machines, in the form of genetically engineered E. coli cells, have solved a variety of mathematical problems, which have had important applications in biology, medicine and technology.
IND-29 Electrophoretic and Chromatographic Analysis of Photosynthetic Pigments from Blue-Green Algae. (View Individual Experiment)
Cyanobactera, also known as blue-green algae, obtain their energy by photosynthesis using sunlight as their energy source. These organisms have been considered to be the oldest and the most important bacteria on the earth. It is believed that they were responsible for the initial oxygenation of the earth's atmosphere through photosynthesis and it is also felt they were the precursors to the chloroplasts that are found in true algae and plants. There are two classes of photosynthetic pigments in Cyanobactera.
This Program is a complete laboratory course for teaching general biology or introductory cell and molecular biology. Like all of our other experiments, the LC6 is designed for 16 students working in pairs. This course provides essentially all of the chemicals and instructions that are needed to teach fifteen, 3-hour laboratory sessions or twenty-five 1-hour laboratory sessions. The course consists of a series of experiments that are presented in a comprehensive integrated laboratory manual which is also provided on a CD in PDF format.
In the first two sections of the course, students study topics in protein biology and biochemistry such as protein structure, function, and isolation. Experiments on enzyme kinetics and cellular metabolism are then carried out. Students perform a project of their own design in the second section of the course. The projects focus on the characterization of plant peroxidases. A number of other optional student-designed experiments are outlined in the Instructor manual of the program and basic reagents are provided in order for the student to carry out their hypothesis driven projects.
Experiments on the properties and structure of DNA are presented in the next section of the course. Here, students perform experiments that deal with genome organization and specific gene function. Techniques include DNA electrophoresis, cell fractionation, DNA isolation, restriction nuclease mapping, and basic cloning procedures. In the final section of the course students study the genetics, biochemistry and molecular biology of hemoglobin.
Recombinant DNA techniques played a central role in the recent emergence of biology into the golden age. This program enables the advanced student to approach these frontiers in biotechnology. The program provides a challenging series of seven 3-hour laboratory sessions which are intended to give students hands-on-experience and a detailed understanding of these new investigative techniques and their potentials.