Molecular Weight Determination (EXP-201P)
A first step in characterizing a protein often involves determining its molecular weight. From this information, different proteins may be compared and the number of amino acid residues in a protein can be determined. Here, students determine the molecular weight of two unknown proteins by comparing their electrophoretic migration with the migration of standard proteins. The protein standards and unknowns have been pre-stained so that your students can follow their
progress during the separation.
Precast polyacrylamide gels can be ordered from Bio Rad, (800-424-6723) Their catalog number is 1611177.