201. Molecular Weight Determination (EXP-201)
Electrophoresis Time (Minutes) Prestained protein standards of known molecular weights and two proteins of unknown molecular weights were subjected to electrophoresis for the indicated times. The molecular weights of the unknown proteins can be determined by comparing their migration with the migration of the standard proteins.
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A first step in characterizing a protein often involves determining its molecular weight. From this information, different proteins may be compared and the number of amino acid residues in a protein can be determined. Here, students determine the molecular weight of two unknown proteins by comparing their electrophoretic migration with the migration of standard proteins. The protein standards and unknowns have been pre-stained so that your students can follow their progress during the separation as shown.