201. Molecular Weight Determination (EXP-201)
ELECTROPHORESIS TIME IN MINUTES. Prestained protein standards of known molecular weights and two proteins of unknown molecular weights were subjected to electrophoresis for the indicated times. (Each of the 4 pictures were taken of the same gel in 5 minute increments.) The molecular weights of the unknown proteins can be determined by comparing their migration with the migration of the standard proteins.
A first step in characterizing a protein often involves determining its molecular weight. From this information, different proteins may be compared and the number of amino acid residues in a protein can be determined. Here, students determine the molecular weight of two unknown proteins by comparing their electrophoretic migration with the migration of standard proteins. The protein standards and unknowns have been pre-stained so that your students can follow their progress during the separation as shown.
Each of the individual experiments is supplied with the chemicals and laboratory guides needed for 16 students working in pairs. If you chose one or more of the experiments from this series, you should also order Electrophoresis Package 2M or Electrophoresis Package 2. Electrophoresis Package 2M provides sufficient agarose, gel stain, and electrophoresis buffers for 1 of the individual experiments in this series (four gels with 15ml of agarose per gel). Electrophoresis Package 2 provides sufficient agarose, gel stain, and electrophoresis buffers for up to 6 of the individual experiments in this series.