1002. Analysis of a Genome Segment (EXP-1002)
Lambda phage DNA (lanes 1, 2, 5, & 6) or a plasmid containing a segment of the phage genome (lanes 3, 4, 7, & 8) were digested with EcoR1 or EcoR1 + BamH1 prior to this electrophoretic separation.
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Phage lambda DNA and a recombinant plasmid containing a segment of the phage lambda genome are provided as the starting point for this exercise. Students digest these DNAs with EcoR1 and BamH1 and then analyze the fragments as shown below. Determination of the size of the fragments enables the student to identify the precise region in the lambda genome that is contained within the plasmid.